This page allows users to get access to all the proteomics level experiments conducted and data generated from Rhizoctonia solani (R. solani) under Sheath Blight (SB) infection condition. Users can download the differentially expressed proteins (DEPs) by clicking on Download the proteins. To know more about MIAPPE, visit https://www.miappe.org/
| Sl.No | Title of the study | Method | Key Findings | Publication | Download the Proteins | MIAPPE_Checklist |
|---|---|---|---|---|---|---|
| Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors | Three strains of R. solani with diffrent host range AG1-IA (monocots), AG3 (dicot) and AG8 (monocot and dicot) were used for analysis. Encoded proteins by AG1-IA and AG8, predicted proteins from GenMark-ES v 2, pedicted genes from AG3 were used for analysis. Secretome similarities was identified using orthofinder v 4.0. CD-HIT (-c 90-n5) was used to cluster similar proteins from all the isolates. Effectors from the isolates were predicted by EffectorP v 1.0. Gene Ontology analysis was done by BlastGO v 6.1. Hirerchial clustering ws done based on 15 criterias. Then plant based AG8 gene expression analysis was done. | A total of 2209 unique proteins were identified from all three isolates. Through TribeMCL, 774 total tribes of proteins were identified where 179 of them has 3 or more members with the largest one. 152 diffrent Pfam domains with CAZy families were identified from AG1-IA. Major effector proteins like Necrosis and Ethylene inducing Proteins (NEPs), Small Cysteine Rich (SCR) , Xylanase and inhibitor l9 were identified which are involved R. solani pathogenesis. | Anderson et al. (2017) | Download | Download | |
| Global Protein?Protein Interaction Network of Rice Sheath Blight Pathogen | This work was started with 10489 AG1-IA specific protein sequences from NCBI. Along with R. solani, interaction datasets of Drosophilla, C.elegans, Human, Yeast and Magneporthe (20207 genes & 59929 interaction) were also used for identification of AG1-IA protein interaction. Domain domain interction (DDI) studies were done by using 5410 domians. Domain annotations were predicted using Pfam with HMMER. Similarly protein protein interaction studies were also done with similar procedures. Generated data were then validated through GO annotation. Yeast two-hybridization assay was performed to see in-cell interaction. | A total of 8312 in protein protein interaction among 1991 R. solani AG1-IA specific genes was predicted. Similarly 1336786 domain domain interactions was identified between 4780 proteins. 3 PPI networks i.e. one core network, one multiple-core network and crossover network was identified which play importatnt roles in pathogenicity. A potentially secreted effector AG1IA_09161 was identified and this interacted with AG1IA_04033 which is thought to be related with synthesis of glycosyphosphatidylinositol. | Lei et al. (2014) | Download | Download | |
| Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation | R. solani AG1-IA strain was grown under dark conditions. Sclerotia of different growth time (3 days, 7 days and 10 days) were isolated and stored. Then they were visualised by SEM folled by protein extraction. the extracted protein were then analysed by SDS-PAGE and 2D-Electrophoresis folled by in gel digestion. The proteins are then identified by MALDI-TOF MS and MALDI-TOF/TOF MS. Then GO analysis was done using TAIR, Uni-PortKB, KEGG and PIR. Then RT-PCR basd analysis was done. | In total, 75 proteins were identified from the experiment which were classified into 10 important categories including metabolism, pathogenicity, toxin production. Especially two proteins related to vacuolar function were identified. | Kwon et al. (2014) | Download | Download |