Research Projects

     On-going Projects

1.    Title: Biodiversity Informatics and Technology Exchange for Snakebite management (BITES) Partner 5 of MARIE CURIE ACTIONS

         International Research Staff Exchange Scheme (IRSES)

                   Coordinator: Dr. Anita Malhotra, Bangor University UK

                   Funded by European Commission

                   Proposal Number: 612131
                   Scientific Panel: LIF
                   Grant Agreement Number: PIRSES-GA-2013-612131
                   Duration of the project: 48 months (Starting Month September 2014)

2.    Title: Isolation, identification and characterization of marker proteins from the Venom of *Naja kaouthia* and *Bungarus fasciatus* of North East India for

         Development of Rapid Diagnostic Tool for Identification of snakebite in North East India.

   Funding Agency:      ICMR, Govt. of India

   Duration:                Three years

   Amount sanctioned: 38.53 lakhs

   Period:                    February 2018-January 2021

      Completed Projects

     1. Title: Search for novel treatments for snake venom poisoning: Composition analysis of venoms of snake family Naja naja and Daboia

                  russelii and complete characterization of their major toxins.

                 Funding Agency:        Department of Biotechnology, Ministry of Science and Technology, Govt. of India

                 Duration:                  Three Years

                 Amount sanctioned:   69.84 Lakhs

                 Period:                      March 2011- September 2014

     2. Title: Isolation, Characterization, possible use and clinical manifestation of a Neurotoxin from Snake venom (Naja siamensis) with
                  special reference to warfare importance neuro- toxin as a model.

                 Funding Agency:        DRDO, Govt. of India

                 Duration:                  Three Years

                 Amount sanctioned:   9.8 Lakhs

                 Period:                      February 2012 - January 2015

      3. Title: Cloning, expression and structural characterization of anti-thrombin from cattle tick salivary gland: designing of novel anti-thrombin peptide

                 Funding Agency:        DST, Govt. of India

                 Duration:                   Four Years

                 Amount sanctioned:   41.06 Lakhs

                 Period:                      July 2014 - June 2018

     [Top]

Research Publications

33. Subhankar N. Choudhury, Barlina Konwar, Simran Kaur, Robin Doley, Biplob Mondal (2018). Study on Snake Venom Protein-Antibody Interaction by Surface Plasmon Resonance Spectroscopy. Photonic Sensors, 1-10

32. Manash Deep Dey, Subhasish Das, Rupesh Kumar, Robin Doley, Satya Sundar Bhattacharya, Rupak Mukhopadhyay (2017). Vermiremoval of methylene blue using Eisenia fetida: A potential strategy for bioremediation of synthetic dye-containing effluents. Ecological Engineering, 106 (2017) 200–208 [IF: 2.914]
 

31. Archana Deka, Maitreyee Sharma, Manoj Sharma, Rupak Mukhopadhyay, Robin Doley (2017). Purification and partial characterization of an anticoagulant PLA₂ from the venom of Indian Daboia russelii that induces inflammation through upregulation of pro- inflammatory mediators. J Biochem Mol Toxicol. 2017 Jun 13 [IF: 1.596]

30. Devabrata Saikia, Ajay Kumar Manhar, Bhagyashree Deka, Raju Roy, Kuldeep Gupta, Nima D. Namsa, Pronobesh Chattopadhyay, Robin Doley, Manabendra Mandal. Hypocholesterolemic activity of indigenous probiotic isolate Saccharomyces cerevisiae ARDMC1 in a rat model. Journal of Food and Drug Analysis Jan 2018, 26(1), 154-162  [IF: 1.98]         

29. Rajeev Kungur Brahma, Guillaume Blanchet, Simran Kaur, R. Manjunatha Kini, Robin Doley. (2017). Expression and characterization of haemathrins, madanin-like thrombin inhibitors, isolated from the salivary gland of tick Haemaphysalis bispinosa (Acari: Ixodidae) Thrombosis Research. Jan 31;152:20-29. [IF: 2.32]

28. Hazarika, Shabiha Nudrat, Kuldeep Gupta, Khan Naseem Ahmed Mohammed Shamin,  Pushpender Bhardwaj, Ratan Boruah, Kamlesh K. Yadav, Ashok Naglot P Deb, M Mandal, Robin Doley, Vijay Veer, Indra Baruah and Nima D Namsa (2016). One-pot facile green synthesis of biocidal silver nanoparticles. Materials Research Express Jul 15;3(7):075401.  [IF: 0.968]

27. Sharma M, Iyer JK, Shih N, Majumder M, Mattaparthi VS, Mukhopadhyay R, Doley R. (2016). Daboxin P, a Major Phospholipase A2 Enzyme from the Indian Daboia russelii russelii Venom Targets Factor X and Factor Xa for Its Anticoagulant Activity. PLoS One. Apr 18;11(4):e0153770. [IF: 3.234]

26. Das HK, Das D, Doley R, Sahu PP. (2016). Quantifying Demyelination in NK venom treated nerve using its electric circuit model. Scientific Reports. Mar 2;6:22385. [IF: 5.578]

25. Das, D., Sharma, M., Das, H., Sahu, P.P and Doley. R (2016). Purification and Characterization of Nk-3FTx, A Three Finger Toxin (3FTx) from the venom of North East Indian Monocled Cobra. Journal of Biochemical and Molecular Toxicology. Feb; 30(2):59-70 [IF: 1.596]

24. Diganta Das, Md. Minhaj Ansari, Nima D. Namsa, Mukherjee, A.K and Robin Doley*  (2015). Assessment of antivenom potential of Anthocephalus cadamba leaves extract: A traditionally used medicinal plants for the treatment of snakebite patients. Journal of Bioresource [IF: Nil] 2(2): 24-32 (2015)

23. Manash Deep Dey, Ruchi Shukla, Naba K. Bordoloi, Robin Doley & Rupak Mukhopadhyay. (2015). Mechanism of Adsorptive Removal of Methylene Blue Using Dried Biomass of Rhizopus oryzae. Applied Biochemistry and Biotechnology Part A: Enzyme Engineering and Biotechnology Sep;177(2):541-55 [IF: 1.735]

22. Maitreyee Sharma, Iyer Janaki Krishnamurthy, R. Manjunatha Kini and Robin Doley (2015). Unveiling the complexities of Daboia russelii venom, a medically important snake of India, by tandem mass spectrometry. Toxicon 107, 266-281[IF: 2.924]

21. Rajeev Kungur Brahma, Ryan J.R. McCleary, R. Manjunatha Kini, Robin Doley (2015). Venom gland transcriptomics for identifying, cataloging, and characterizing venom proteins in snakes Toxicon 93, 1–10. [IF: 2.924]

20. Kunal Biswas, Jetendra Kumar Roy, Robin Doley, Dambarudhar Mohanta, and Ashish   Kumar Mukherjee (2014). Imaging Bactericidal Effect of Faceted Ag Nanostructures (FAgN) on Gram Negative, Coli Form Escherichia coli Bacteria J. Bionanosci. 8, 248-254. [IF: Nil]

19. Kalita, D, Saikia, J, Mukherjee, AK and Doley, R (2014). An ethnomedicinal survey of traditionally used medicinal plants for the treatment of snakebite in Morigaon district of Assam, India. International Journal of Medicinal and Aromatic Plants, Vol. 4, No. 2, pp. 97-106. (Figure in cover page). [IF: Nil]

18. Brahma RK, Dixit V, Sangwan AK, Doley R (2014). Identification and characterization of Rhipicephalus (Boophilus) microplus and Haemaphysalis bispinosa ticks (Acari: Ixodidae) of North East India by ITS2 and 16S rDNA sequences and morphological analysis. Exp Appl Acarol. 62:253-265 [IF: 1.821]

17. Maitreyee Sharma, Neeharika Gogoi, B. L. Dhananjaya, Jaideep C. Menon, and Robin Doley (2014). Geographical variation of Indian Russell’s viper venom and neutralization of its coagulopathy by polyvalent antivenom. J. Toxicol. Toxin. Rev. Vol. 33, No. 1-2: 7–15. [IF: 0.842]

16. Diganta Das, Nanjaraj Urs, Vilas Hiremath, Bannikuppe Sannanaik Vishwanath and Robin Doley. (2013). Biochemical and Biological Characterization of Naja kaouthia venom from North-East India and its Neutralization by Polyvalent antivenom. Journal of Venom Research Vol 4, 31-38. [IF: Nil]

15. Anjan Barman, Praveen Kumar, Vinay Kumar, Robin Doley and Suvendra Kumar Ray (2013). Heat shock at 37°C with plasmid ligated at 37°C yields more number of Escherichia coli transformants than plasmid ligated at 16°C: a possible role of ligated plasmid conformation during heat shock. Current Science, (747), March, 104 (06). [IF: 0.833]

14. Freek J. Vonk, Kate Jackson, Robin Doley, Frank Madaras, Peter J. Mirtschin and Nicolas Vidal. (2011). Snake venom: From fieldwork to the clinic: Recent insights into snake biology, together with new technology allowing high throughput screening of venom, bring new hope for drug discovery. BioEassys Apr;33(4):269-79. [IF: 4.838]

13. Kini R.M and Doley R. (2010). Structure, Function and Evolution of Three-Finger Toxins – Mini Proteins with Multiple Targets. Toxicon. Nov;56(6):855-67 [IF: 2.924] (Highly cited)

12. Doley, R, Susanta Pahari, Md Abu Reza1, Stephen P. Mackessy and R. Manjunatha Kini. (2010). The Gene Structure and Evolution of ku-wap-fusin (Kunitz Waprin Fusion Protein), a Novel Evolutionary Intermediate of the Kunitz Serine Protease Inhibitors and Waprins from Sistrurus catenatus (Massasauga Rattlesnake) Venom Glands. The Open Evolution Journal, 4, 31-41. [IF: Nil]

11. Ang Swee Siang, Doley, R Freek J. Vonk and Kini, R.M. (2010). Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags. BMC Molecular Biology, 11 (1) :24 [IF: 2.796]

10. Ompraba G, Chapeaurouge A, Doley R, Devi RK, Padmanaban P, Venkatraman C, Velmurugan D, Qingsong L, and Kini RM. (2010). Identification of a novel family of snake venom proteins veficolins from Cerberus rynchops using a venom gland transcriptomics and proteomics approach. J Proteome Res. 5;9(4):1882-93. [IF: 5.056]

9. Cassandra M Modahl, Robin Doley and R. Manjunatha Kini. (2010). Venom Analysis of Long-Term Captive Pakistan Cobra (Naja naja) Populations. Toxicon. Vo. 55, Issues 2-3, 612-618. [IF: 2.924]

8. Doley, R. and Kini RM. (2009). Protein Complexes in Snake Venom. Cell Mol. Life. Sci (Cellular and Molecular Life Sciences) 66(17): 2851-71. [IF: 5.856]

7. Doley, R., Mackessy, S.P and Kini RM. (2009). Role of Accelerated Segment Switch in Exon to alter Targeting (ASSET) in Molecular Evolution of Snake Venom Proteins. BMC Evolutionary Biology. 9:146 [IF: 3.047]

6. Doley, R., Pahari, S., Mackessy, S.P and Kini R.M (2008). Accelerated exchange of exon segments in three-finger toxin genes of Sistrurus catenatus edwardsii (Desert Massasauga; Viperidae) Venom. BMC Evolutionary Biology.  8:196 [IF: 3.047]

5. Doley, R., Tram, N.N., Reza M.A and Kini, R.M (2008). Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from Kangaroo Island. BMC Evolutionary Biology.  8:70 [IF: 3.047]

4. Ashis K. Mukherjee, Robin Doley and Debashree Saikia (2008). Isolation of a snake venom phospholipase A₂ (PLA₂) inhibitor (AIPLAI) from leaves of Azadirachta indica (Neem): Mechanism of PLA₂ inhibition by AIPLAI in-vitro condition. Toxicon. 51 (8), 1548-1553. [IF: 2.924]

3. Doley, R., King, G.F. and Mukherjee, A.K. (2004). Differential hydrolysis of erythrocyte and mitochondrial membrane phospholipids by two phospholipase A2 isoenzymes (NK-PLA2-I and NK-PLA2-II), from Indian monocled cobra Naja kaouthia venom. Archives of Biochemistry and Biophysics. 425 (1): 1-13. [IF: 3.043]

2. Das, K., Doley, R and Mukherjee, A.K. (2004). Purification and biochemical characterization of a thermostable, alkaliphilic, extracellular α-amylase from Bacillus subtilis DM-03, isolated from the traditional fermented food of India. Biotechnology Applied Biochemistry. 40 (Pt 3): 291-198. [IF: 1.322]

1. Doley, R. and Mukherjee, A.K. (2003). Purification and characterization of an anticoagulant phospholipase A2 from Indian monocled cobra (Naja kaouthia) venom. Toxicon. 41 (1):81-91.[IF:2.924]
 

Book Chapters:

  1. Robin Doley, Xingding Zhou and R. Manjunatha Kini. Snake Venom Phospholipase A₂ Enzymes In: Handbook of Venoms and Toxins Reptiles Edited by Stephen

      P.  Mackessy. CRC Press/Taylor and Francis.

       2. Raghurama Hedge, Rajagopalan Nandakishore, Robin Doley, and R. Manjunatha Kini. Snake Venom Three-finger Toxins In: Handbook of Venoms and Toxins of

             Reptiles Edited by Stephen P. Mackessy. CRC Press/Taylor and Francis.

       3. Doley, R. and Mukherjee, A.K. (2003) Pharmacological assessment of medicinal plants to ascertain their anti-snake venom activity. In: Ethnomedicine of North-East

             India: Protection, Utilization and Conservation. (Editors: Singh, G, Singh, H.B. and Mukherjee, T.K), National Institute of Science Communication and Information   

           Resources, CSIR, New Delhi, pp: 300-304