Molecular Toxinology Laboratory

Department of Molecular Biology and Biotechnology

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Robin Doley, PhD

Associate Professor & Principal Investigator

Research Interest:


Snake venom is a complex mixture of bioactive proteins and polypeptides produced and stored in the venom gland. They are responsible for the pharmacological manifestation in victims during envenomation by venomous snakes. Variation in the venom composition adds more to the complexities of pharmacological effects. Venom composition of single species varies due to age, sex, geographical locations and others. Due to this antivenoms available are ineffective in neutralizing all the toxic components of the venom. Therefore understanding the venom variation and bioactivity of these proteins and polypeptides is very important. This will help in designing strategies to overcome the limitations of antivenom therapy but also lead to drug discoveries. Therefore our research interest are 1) Understand variation of snake venoms proteins from different geographical locations, 2) Venomics and antivenomics of medically important snakes of India, 3) Understanding the structure-function relationship of these venom proteins for therapeutic applications.


Research Projects                   Research Group            Lab Alumni               Publications         Google Scholar           Contact Address

Personal Details Principal Investigator

Postdoc. (2005-2009): Protein Science Laboratory

                                   Dept. of Biological Sciences

                                   National University of Singapore, Singapore


  PhD (2004):               Dept. of Molecular Biology and Biotechnology

                                   Tezpur University, Assam, India


  M.Sc. (1999)            Dept. of Zoology

                                   Rajiv Gandhi University, Doimukh, Arunachal Pradesh, India



Position Held

 Associate Professor, Department of Molecular Biology and Biotechnology, Tezpur University, February 22, 2013 till date

 Assistant Professor, Department of Molecular Biology and Biotechnology, Tezpur University, October 2009 to February 22, 2013

 Lecturer (Adhoc), Department of Molecular Biology and Biotechnology, Tezpur University, March 2009 to September 2009.

 Post Doctoral Fellow, National University of Singapore, March 2005 to March 2009

 UGC Research Fellow, 2001 for pursuing doctoral research


Membership/Academic Distinctions / Honors / Awards

 Life member: Toxinological Society of India

 Life Member: Society of Biological Chemist (India)

 Member: International Society on Thrombosis and Haemostasis (ISTH)

 Member: International Society on Toxinology (IST)

 CSIR-SRF: 2003

 Chancellor’s Gold Medal, Rajiv Gandhi University: 1999

 Gold Medal in PG, Rajiv Gandhi University: 1999


  Present and Past Administrative/Academic Assignments

  Member: Internal Quality Assurance Cell (IQAC) (2015-17)

  Reviewing Editor: Journal of Bioresource (ISSN 2394-4315)

  Member Secretary: Tezpur University Animal Ethical Committee (TUAEC)

  Member: Institutional Animal Ethical Committee (IAEC), DRL, Tezpur

  Member: Institutional Biosafety Committee (IBC), DRL, Tezpur

  Member: Institutional Biosafety Committee, Tezpur University

  Member: Sophisticated Instrument Facility, Tezpur University

  Member: Core Committee, Tezpur University Jonai Campus

  Associate editor, Newsletter, Toxinological Society of India, 2010 till 2014

  Warden, New Women's Hostel, Tezpur University, August 2012 to July 2013

  Warden, Brahmaputra Men's Hostel, Tezpur University, July 2010 to Aug 2012



Resource person

  1. CEP on Ethanobotany and Folk Medicine: Exploration of Ethanomedicine of North-East India, DRL, Tezpur, December 12-16, 2011.

  2. Biotech-Hub Workshop on Basic Genetic Engineering Techniques for Gene Cloning” Organized by Department of Molecular Biology and Biotechnology, Tezpur University.

      December 17-21, 2012.

  3. Career Oriented Talk on Biotechnology, Darrang College, 17 Aug. 2013

  4. Workshop on Molecular Structure Determination using X-ray Crystallography: Theory and Hands on Experience, Centre for Potential for Excellence in Biodiversity,

      Rajiv Gandhi University, 4-6 Sept. 2013

  5. DBT sponsored workshop on "Techniques of Molecular Biology". Organized by Institutional Level Biotech Hub (IBT Hub), A. D. P College, Nagaon 752002, Assam.

      May 30-31, 2014

  6. DBT sponsored workshop on "Biochemistry and Molecular Biology Techniques". Centre for Studies in Biotechnology Dibrugarh University, Dibrugarh, Assam.

      March 19-21, 2014

  7. CEP course on "Advances in biotechnology and its impact on human health DRL, Tezpur, November 7-11, 2016.



       Research Projects

     On-going Projects


1.    Title: Biodiversity Informatics and Technology Exchange for Snakebite management (BITES) Partner 5 of MARIE CURIE ACTIONS

         International Research Staff Exchange Scheme (IRSES)

                   Coordinator: Dr. Anita Malhotra, Bangor University UK

                   Funded by European Commission

                   Proposal Number: 612131
                   Scientific Panel: LIF
                   Grant Agreement Number: PIRSES-GA-2013-612131
                   Duration of the project: 48 months (Starting Month September 2014)


2.    Title: Isolation, identification and characterization of marker proteins from the Venom of *Naja kaouthia* and *Bungarus fasciatus* of North East India for

         Development of Rapid Diagnostic Tool for Identification of snakebite in North East India.

   Funding Agency:      ICMR, Govt. of India

   Duration:                Three years

   Amount sanctioned: 38.53 lakhs

   Period:                    February 2018-January 2021



      Completed Projects

     1. Title: Search for novel treatments for snake venom poisoning: Composition analysis of venoms of snake family Naja naja and Daboia

                  russelii and complete characterization of their major toxins.

                 Funding Agency:        Department of Biotechnology, Ministry of Science and Technology, Govt. of India

                 Duration:                  Three Years

                 Amount sanctioned:   69.84 Lakhs

                 Period:                      March 2011- September 2014



     2. Title: Isolation, Characterization, possible use and clinical manifestation of a Neurotoxin from Snake venom (Naja siamensis) with
                  special reference to warfare importance neuro- toxin as a model.

                 Funding Agency:        DRDO, Govt. of India

                 Duration:                  Three Years

                 Amount sanctioned:   9.8 Lakhs

                 Period:                      February 2012 - January 2015


      3. Title: Cloning, expression and structural characterization of anti-thrombin from cattle tick salivary gland: designing of novel anti-thrombin peptide

                 Funding Agency:        DST, Govt. of India

                 Duration:                   Four Years

                 Amount sanctioned:   41.06 Lakhs

                 Period:                      July 2014 - June 2018


Research Publications

33. Subhankar N. Choudhury, Barlina Konwar, Simran Kaur, Robin Doley, Biplob Mondal (2018). Study on Snake Venom Protein-Antibody Interaction by Surface Plasmon Resonance Spectroscopy. Photonic Sensors, 1-10

32. Manash Deep Dey, Subhasish Das, Rupesh Kumar, Robin Doley, Satya Sundar Bhattacharya, Rupak Mukhopadhyay (2017). Vermiremoval of methylene blue using Eisenia fetida: A potential strategy for bioremediation of synthetic dye-containing effluents. Ecological Engineering, 106 (2017) 200–208 [IF: 2.914]

31. Archana Deka, Maitreyee Sharma, Manoj Sharma, Rupak Mukhopadhyay, Robin Doley (2017). Purification and partial characterization of an anticoagulant PLA2 from the venom of Indian Daboia russelii that induces inflammation through upregulation of pro- inflammatory mediators. J Biochem Mol Toxicol. 2017 Jun 13 [IF: 1.596]


30. Devabrata Saikia, Ajay Kumar Manhar, Bhagyashree Deka, Raju Roy, Kuldeep Gupta, Nima D. Namsa, Pronobesh Chattopadhyay, Robin Doley, Manabendra Mandal. Hypocholesterolemic activity of indigenous probiotic isolate Saccharomyces cerevisiae ARDMC1 in a rat model. Journal of Food and Drug Analysis Jan 2018, 26(1), 154-162  [IF: 1.98]         


29. Rajeev Kungur Brahma, Guillaume Blanchet, Simran Kaur, R. Manjunatha Kini, Robin Doley. (2017). Expression and characterization of haemathrins, madanin-like thrombin inhibitors, isolated from the salivary gland of tick Haemaphysalis bispinosa (Acari: Ixodidae) Thrombosis Research. Jan 31;152:20-29. [IF: 2.32]


28. Hazarika, Shabiha Nudrat, Kuldeep Gupta, Khan Naseem Ahmed Mohammed Shamin,  Pushpender Bhardwaj, Ratan Boruah, Kamlesh K. Yadav, Ashok Naglot P Deb, M Mandal, Robin Doley, Vijay Veer, Indra Baruah and Nima D Namsa (2016). One-pot facile green synthesis of biocidal silver nanoparticles. Materials Research Express Jul 15;3(7):075401.  [IF: 0.968]


27. Sharma M, Iyer JK, Shih N, Majumder M, Mattaparthi VS, Mukhopadhyay R, Doley R(2016). Daboxin P, a Major Phospholipase A2 Enzyme from the Indian Daboia russelii russelii Venom Targets Factor X and Factor Xa for Its Anticoagulant Activity. PLoS One. Apr 18;11(4):e0153770. [IF: 3.234]


26. Das HK, Das D, Doley R, Sahu PP. (2016). Quantifying Demyelination in NK venom treated nerve using its electric circuit model. Scientific Reports. Mar 2;6:22385. [IF: 5.578]


25. Das, D., Sharma, M., Das, H., Sahu, P.P and Doley. R (2016). Purification and Characterization of Nk-3FTx, A Three Finger Toxin (3FTx) from the venom of North East Indian Monocled Cobra. Journal of Biochemical and Molecular Toxicology. Feb; 30(2):59-70 [IF: 1.596]


24. Diganta Das, Md. Minhaj Ansari, Nima D. Namsa, Mukherjee, A.K and Robin Doley*  (2015). Assessment of antivenom potential of Anthocephalus cadamba leaves extract: A traditionally used medicinal plants for the treatment of snakebite patients. Journal of Bioresource [IF: Nil] 2(2): 24-32 (2015)


23. Manash Deep Dey, Ruchi Shukla, Naba K. Bordoloi, Robin Doley & Rupak Mukhopadhyay. (2015). Mechanism of Adsorptive Removal of Methylene Blue Using Dried Biomass of Rhizopus oryzae. Applied Biochemistry and Biotechnology Part A: Enzyme Engineering and Biotechnology Sep;177(2):541-55 [IF: 1.735]


22. Maitreyee Sharma, Iyer Janaki Krishnamurthy, R. Manjunatha Kini and Robin Doley (2015). Unveiling the complexities of Daboia russelii venom, a medically important snake of India, by tandem mass spectrometry. Toxicon 107, 266-281[IF: 2.924]


21. Rajeev Kungur Brahma, Ryan J.R. McCleary, R. Manjunatha Kini, Robin Doley (2015). Venom gland transcriptomics for identifying, cataloging, and characterizing venom proteins in snakes Toxicon 93, 1–10. [IF: 2.924]


20. Kunal Biswas, Jetendra Kumar Roy, Robin Doley, Dambarudhar Mohanta, and Ashish   Kumar Mukherjee (2014). Imaging Bactericidal Effect of Faceted Ag Nanostructures (FAgN) on Gram Negative, Coli Form Escherichia coli Bacteria J. Bionanosci. 8, 248-254. [IF: Nil]


19. Kalita, D, Saikia, J, Mukherjee, AK and Doley, R (2014). An ethnomedicinal survey of traditionally used medicinal plants for the treatment of snakebite in Morigaon district of Assam, India. International Journal of Medicinal and Aromatic Plants, Vol. 4, No. 2, pp. 97-106. (Figure in cover page). [IF: Nil]


18. Brahma RK, Dixit V, Sangwan AK, Doley R (2014). Identification and characterization of Rhipicephalus (Boophilus) microplus and Haemaphysalis bispinosa ticks (Acari: Ixodidae) of North East India by ITS2 and 16S rDNA sequences and morphological analysis. Exp Appl Acarol. 62:253-265 [IF: 1.821]


17. Maitreyee Sharma, Neeharika Gogoi, B. L. Dhananjaya, Jaideep C. Menon, and Robin Doley (2014). Geographical variation of Indian Russell’s viper venom and neutralization of its coagulopathy by polyvalent antivenom. J. Toxicol. Toxin. Rev. Vol. 33, No. 1-2: 7–15. [IF: 0.842]


16. Diganta Das, Nanjaraj Urs, Vilas Hiremath, Bannikuppe Sannanaik Vishwanath and Robin Doley. (2013). Biochemical and Biological Characterization of Naja kaouthia venom from North-East India and its Neutralization by Polyvalent antivenom. Journal of Venom Research Vol 4, 31-38. [IF: Nil]


15. Anjan Barman, Praveen Kumar, Vinay Kumar, Robin Doley and Suvendra Kumar Ray (2013). Heat shock at 37°C with plasmid ligated at 37°C yields more number of Escherichia coli transformants than plasmid ligated at 16°C: a possible role of ligated plasmid conformation during heat shock. Current Science, (747), March, 104 (06). [IF: 0.833]


14. Freek J. Vonk, Kate Jackson, Robin Doley, Frank Madaras, Peter J. Mirtschin and Nicolas Vidal. (2011). Snake venom: From fieldwork to the clinic: Recent insights into snake biology, together with new technology allowing high throughput screening of venom, bring new hope for drug discovery. BioEassys Apr;33(4):269-79. [IF: 4.838]


13. Kini R.M and Doley R. (2010). Structure, Function and Evolution of Three-Finger Toxins – Mini Proteins with Multiple Targets. Toxicon. Nov;56(6):855-67 [IF: 2.924] (Highly cited)


12. Doley, R, Susanta Pahari, Md Abu Reza1, Stephen P. Mackessy and R. Manjunatha Kini. (2010). The Gene Structure and Evolution of ku-wap-fusin (Kunitz Waprin Fusion Protein), a Novel Evolutionary Intermediate of the Kunitz Serine Protease Inhibitors and Waprins from Sistrurus catenatus (Massasauga Rattlesnake) Venom Glands. The Open Evolution Journal, 4, 31-41. [IF: Nil]


11. Ang Swee Siang, Doley, R Freek J. Vonk and Kini, R.M. (2010). Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags. BMC Molecular Biology, 11 (1) :24 [IF: 2.796]


10. Ompraba G, Chapeaurouge A, Doley R, Devi RK, Padmanaban P, Venkatraman C, Velmurugan D, Qingsong L, and Kini RM. (2010). Identification of a novel family of snake venom proteins veficolins from Cerberus rynchops using a venom gland transcriptomics and proteomics approach. J Proteome Res. 5;9(4):1882-93. [IF: 5.056]


9. Cassandra M Modahl, Robin Doley and R. Manjunatha Kini. (2010). Venom Analysis of Long-Term Captive Pakistan Cobra (Naja naja) Populations. Toxicon. Vo. 55, Issues 2-3, 612-618. [IF: 2.924]


8. Doley, R. and Kini RM. (2009). Protein Complexes in Snake Venom. Cell Mol. Life. Sci (Cellular and Molecular Life Sciences) 66(17): 2851-71. [IF: 5.856]


7. Doley, R., Mackessy, S.P and Kini RM. (2009). Role of Accelerated Segment Switch in Exon to alter Targeting (ASSET) in Molecular Evolution of Snake Venom Proteins. BMC Evolutionary Biology. 9:146 [IF: 3.047]


6. Doley, R., Pahari, S., Mackessy, S.P and Kini R.M (2008). Accelerated exchange of exon segments in three-finger toxin genes of Sistrurus catenatus edwardsii (Desert Massasauga; Viperidae) Venom. BMC Evolutionary Biology.  8:196 [IF: 3.047]


5. Doley, R., Tram, N.N., Reza M.A and Kini, R.M (2008). Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from Kangaroo Island. BMC Evolutionary Biology.  8:70 [IF: 3.047]


4. Ashis K. Mukherjee, Robin Doley and Debashree Saikia (2008). Isolation of a snake venom phospholipase A2 (PLA2) inhibitor (AIPLAI) from leaves of Azadirachta indica (Neem): Mechanism of PLA2 inhibition by AIPLAI in-vitro condition. Toxicon. 51 (8), 1548-1553. [IF: 2.924]


3. Doley, R., King, G.F. and Mukherjee, A.K. (2004). Differential hydrolysis of erythrocyte and mitochondrial membrane phospholipids by two phospholipase A2 isoenzymes (NK-PLA2-I and NK-PLA2-II), from Indian monocled cobra Naja kaouthia venom. Archives of Biochemistry and Biophysics. 425 (1): 1-13. [IF: 3.043]


2. Das, K., Doley, R and Mukherjee, A.K. (2004). Purification and biochemical characterization of a thermostable, alkaliphilic, extracellular α-amylase from Bacillus subtilis DM-03, isolated from the traditional fermented food of India. Biotechnology Applied Biochemistry. 40 (Pt 3): 291-198. [IF: 1.322]


1. Doley, R. and Mukherjee, A.K. (2003). Purification and characterization of an anticoagulant phospholipase A2 from Indian monocled cobra (Naja kaouthia) venom. Toxicon. 41 (1):81-91.[IF:2.924]


Book Chapters:


  1. Robin Doley, Xingding Zhou and R. Manjunatha Kini. Snake Venom Phospholipase A2 Enzymes In: Handbook of Venoms and Toxins Reptiles Edited by Stephen

      P.  Mackessy. CRC Press/Taylor and Francis.


       2. Raghurama Hedge, Rajagopalan Nandakishore, Robin Doley, and R. Manjunatha Kini. Snake Venom Three-finger Toxins In: Handbook of Venoms and Toxins of

             Reptiles Edited by Stephen P. Mackessy. CRC Press/Taylor and Francis.


       3. Doley, R. and Mukherjee, A.K. (2003) Pharmacological assessment of medicinal plants to ascertain their anti-snake venom activity. In: Ethnomedicine of North-East

             India: Protection, Utilization and Conservation. (Editors: Singh, G, Singh, H.B. and Mukherjee, T.K), National Institute of Science Communication and Information   

           Resources, CSIR, New Delhi, pp: 300-304


      Research Group

Description: Description: Description: Description: Description: Description: C:\Users\acer\Desktop\RD_new2\Simran.jpg 

Simran Kaur

DBT-JRF (2014), GATE (2013 & 14), SLET (2013)

Ph. D Scholar (TZ121012)


Area of research: Cloning and expression of venom proteins


Archana Deka

DBT-JRF (2016), UGC-NET (2016), ICMR (2016)

GATE (2015), SLET (2014)

Ph. D Scholar (TZ110462)


Area of research: Venomics and Antivenomics




Rafika Yasmin

UGC-NET (2016), UGC-JRF (2016), GATE (2017),

SLET (2018)

Ph. D Scholar (TZ155926)


Area of research: Anti-platelet proteins from snake venom



Susmita Thakur

GATE (2018), SLET (2018)

Ph. D Scholar


Area of research: Venomics and Antivenomics


Arpita Devi

UGC-NET (2015), CSIR-JRF (2016 & 2017),

GATE (2018)

Ph. D Scholar


Area of research: Design and development of anti-thrombin peptides


Amit Talukdar

CSIR NET JRF (2017), SLET (2017)

Ph.D Scholar








      Lab Alumni

      PhD Students

Title of the PhD Thesis

 Present Occupation

      1. Dr. Diganta Das (2010-16)

Studies on the crude venom and purified three-finger toxin of Naja kaouthia

from North East India

  DBT-Post Doc Fellow @ IISc Bangalore


     2.  Dr. Maitreyee Sharma

          DST INSPIRE Fellow  (2011-16)


Proteomics of Indian Daboia russelii venom (Irula) and characterization

of a major protein


  DBT-Post Doc Fellow @ CCMB Hyderabad


      3. Dr. Rajeev Kungur Brahma

          DBT-SRF (2011-16)


Identification and characterization of thrombin inhibitors from the salivary

gland of tick (Haemaphysalis bispinosa)


  Research Associate @ DRL, Tezpur 


      Project Students


Present Occupation

      1.  Rajeev K. Brahma (MSc 2010)

Research Associate, DRL, Tezpur 

      2.  Anoop S. Chauhan (MSc 2011)

PhD, IMTECH Chandigarh 

      3.  Shatabdi Bhattarcharjee (MSc 2011

PhD, Johns Hopkins Bloomberg School of Public Health, USA

      4.  Vanlalrinchhani (MSc 2011)

PhD, IISc Bangalore

      5.  Sharanya Chatterjee (MSc 2012)

PhD, IISc Bangalore

      6.  Moushumee Das (MSc 2012)

PhD, IIT Guwahati

      7.  Simran Kaur (MSc 2012)

PhD, Tezpur University

      8.  Raka Choudhury (MSc 2013)

PhD, Wgeningen University, Netherlands

      9.  Vishal Dixit (MSc 2013)

PhD, Centre for Molecular Medicine, University of Cologne, Germany

      10. Aniket Girish Parab (MSc 2014)

PhD, IISc Bangalore

      11. Salik Miskat Borbora (MSc 2014)

PhD, IISc Bangalore

      12. Archana Deka (Int. MSc 2014)

PhD, Tezpur University

      13. Sharmistha Debnath (Int. MSc 2015)

Biotech Job, Bangalore

      14. Yash Verma (MSc 2015)

PhD, Delhi University

      15. Sumeet Jhariya (MSc 2016)


      16. Rituparna Das (MSc 2016)


17. Debarun Patra (MSc 2017)

18. Rafika Yasmin (MSc 2017)

PhD, Tezpur University

19. Mayashree Das (Int. MSc 2017)

PhD, IISc Bangalore

20. Jebin Ahmed (Int. MSc 2018)


21. Sangeeta Paul (MSc 2018)

PhD, IGIB, Delhi

22. Bhaskar Jyoti Saikia (MSc 2018)

Teaching Job


JRF/Project Fellows

1. Neeharika Gogoi

2. Debajit Kalita

3. Palakshi Das
4. Md. Minhaj Ansari

5. Jotin Gogoi

6. Arpita Devi

7. Amit Talukdar

PhD, NEHU, Shillong

PhD, NEHU, Shillong



PhD, CCMB, Hyderabad

PhD, Tezpur University

PhD, Tezpur University




      Summer/Winter Trainees trained

Sl. No

















Sumita Dutta                          

Shama Hasrat                        

Swagata Sharma                    

Monoj Kr. Das                       

Megha Lekharu                    

Chayanika Sarmah                

Rimpi Saikia                        


Chandrika Konwar                

Pinakshi Gohain                    

Sheetal Ramani                    

Taniya Talwar                       

Pinku Mani Devi      














Cotton College, Guwahati, Assam, India

Jaipur National University, India

SRM Institute of Science and Technology, Tamil Nadu, India

Tezpur University, Tezpur

Cotton College, Guwahati

Cotton College, Guwahati

Tezpur University, Tezpur

Lovely Professional University, Jalandhar

Daulat Ram College, University of Delhi  

SRM Institute of Science and Technology, Tamil Nadu, India  

Tezpur University, Tezpur

Pragjyotish College, Guwahati

Pragjyotish College, Guwahati


Malaya Mili


Tezpur University, Tezpur


Sagar Mazumdar


Tezpur University, Tezpur


Contact Address

Associate Professor

Department of Molecular Biology and Biotechnology

Tezpur University

Tezpur   784 028, Assam, India

Contact: +91 3712 27 5412

Email: doley(AT); doleyr(AT)    

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